Effects of wogonin on the proliferation and migration of nasopharyngeal carcinoma cells

Abstract

Objective To investigate the effects of wogonin on the proliferation and migration of nasopharyngeal carcinoma cells C666-1 and HK-1. Methods The inhibitory effect of wogonin on nasopharyngeal carcinoma cells C666-1 and HK-1 was determined by CCK8 assay, and IC50 was calculated. The effects of wogonin on the proliferation and independent viability of nasopharyngeal carcinoma cell C666-1 were investigated by plate colony formation test. The effects of wogonin on the migration ability of nasopharyngeal carcinoma cells were studied by scratch test and Transwell migration test. Results The results of CCK8 experiment showed that wogonin significantly inhibited the activity of C666-1 and HK-1 cells, and the inhibitory effect increased with the increase of drug concentration. Under the same drug concentration, the inhibitory effect for 48 h was higher than that for 24 h, and the inhibitory effect was dose-time dependent. The IC50 values of wogonin treated C666-1 cells were 37.15 μmol/L for 24 h and 20.75 μmol/L for 48 h. The IC50 values of HK-1 cells treated with wogonin were 29.09 μmol/L for 24 h and 20.46 μmol/L for 48 h. The results of plate colony experiment showed that after the intervention of wogonin, the number of visible colonies of nasopharyngeal carcinoma cells C666-1 was less and the volume was smaller. Scratch test and Transwell migration test showed that the migration ability of C666-1 and HK-1 cells was significantly inhibited, and the higher the drug concentration, the stronger the inhibition effect. Conclusion Wogonin can inhibit the activity, proliferation and migration of nasopharyngeal carcinoma cells.